MB040-HY2: 2x HY PCR Master Mix, with blue dye
* The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
* The high yield PCR Master Mix has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.
* The tracking dye and precipitant have been added into the PCR PreMix so that the PCR product can be directly loaded for electrophoresis.
Features of the blend of Taq enzyme and a proofreading enzyme contained in the high yield PCR master mix:
* Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Proofreading with 5′ exonuclease activity.MB016-HYT: High Yield Taq DNA Polymerase
* Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the high yield Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Proofreading with 5′ exonuclease activity.MB118: Hot-Start High-Fidelity DNA Polymerase
* Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
* Fast amplification speed: normally ~30 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional Taq DNA polymerase.
* High specificity: Its amplification mismatch rate is 1/53 that of ordinary Taq polymerase and 1/6 that of Pfu polymerase.
* Reproducible results: even with the GC-rich fragments and in the presence of PCR inhibitors.
* Blunt-ended amplified products.
* Low cost: $0.8 per unit. The cheapest hot start high fidelity Taq enzyme in the market.MB087: Hot Start Taq DNA Polymerase
* High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
* High specificity.
* Reproducible results.
* Terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product, facilitating TA cloning of PCR products.
* Low cost: $0.19+ per unit. The cheapest hot start Taq enzyme in the market.MB117: Hot Start Taq DNA Polymerase for Probe-based qPCR
* High amplification specificity and sensitivity for detection of low-copy genes paired with the most suitable buffer optimized for qPCR and PCR.
* For probe-based qPCR, excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes.
* Compatibility in terms of template type, template GC content, and primer Tm values.
* Good tolerance to impurities and is suitable for use in a variety of testing scenarios.
* Low cost: $0.19+ per unit. The cheapest hot start Taq enzyme in the market.MB040: GC rich PCR Enhancer
The GC rich PCR enhancer can improve the efficiency of PCR amplification of GC rich DNA templates, increase the specificity of PCR products, and reduce non-specific and undesirable PCR products.MB042-EUT: Taq DNA Polymerase
* High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product, facilitating TA cloning of PCR products.
* Low cost: 2.8+ cents per unit. The cheapest PCR enzyme in the market.